2 x Doktorarbeit
The lab is interested in signal transduction pathways regulating different aspects of muscle stem cells, myogenesis and formation/maintenance of neuromuscular synapses.
Selected Previous Work
Huraskin et al, Development, 2016
Here, we elucidated the role of canonical Wnt signaling activity in adult muscle fibers using a well-established Axin2-lacZ reporter mouse paradigm. In these mice, canonical Wnt signaling is reflected by lacZ expression under control of the endogenous Axin2 promoter. We detected active canonical Wnt signaling (1) in myotubes derived from cultured C2C12 cells or murine primary myoblasts, (2) in muscle fibers of type IIa and, most likely, type IIx, (3) at neuromuscular synapses and (4) during regeneration of skeletal muscle after injury. Interestingly, YAP/Taz/Tead1-mediated signaling accompanied canonical Wnt signaling in adult muscle fibers.
Kravic et al., Autophagy, 2018
Here, we asked whether CSNK2 phosphorylates TOMM22 in skeletal muscle fibers of mice and whether this influences mitochondrial physiology. We show that (1) CSNK2 phosphorylates TOMM22, (2) CSNK2-dependent TOMM22 phosphorylation is not involved in the regulation of mitochondrial protein import in vitro, (3) skeletal muscles from skeletal muscle Csnk2-beta cKO mice contain dysfunctional mitochondria, and (4) mitophagy markers are involved in the removal of impaired mitochondria.
The projects in the lab deal with better understanding of molecular pathways in myogenesis in health and disease. To this end, we use genetically modified mice or primary cultured muscle stem cells. We analyze muscle phenotypes of mutant mice by their behavior, grip strength measurements, and histological / histochemical / immunostainings of muscle sections. We investigate muscle cells and neuromuscular synapses by structural and functional approaches, like 3D morphometrical imaging, and electrophysiological recordings of neuromuscular transmission. Muscular target genes of signaling pathways will be analyzed by ‘omics’ strategies, like RNA-Seq and proteome analysis. Altogether, our projects help to understand individual and concerted roles of intracellular signaling pathways regarding neuromuscular biology.
You should be a young motivated scientist with excellent marks and willing to use this opportunity for your scientific career. Our lab is speaking english and german language. PhD student candidates should have a degree in a relevant field.
How to apply
Please send your application as a single PDF file by E-Mail.